RBS

Part:BBa_K4271006:Design

Designed by: Ethan Ho   Group: iGEM22_Wego_Taipei   (2022-09-24)


pNP RBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal SpeI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 16
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal SpeI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal SpeI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

n/a


Source

engineered RBS from Jha et. al https://doi.org/10.1093/nar/gkw687


References

Jha, R. K., Kern, T. L., Kim, Y., Tesar, C., Jedrzejczak, R., Joachimiak, A., & Strauss, C. (2016, August 17). A microbial sensor for organophosphate hydrolysis exploiting an engineered specificity switch in a transcription factor. Nucleic Acids Research, 44(17), 8490–8500. https://doi.org/10.1093/nar/gkw687